Oral Presentation ESA-SRB 2023 in conjunction with ENSA

Glucagon stimulation test: clinical and biochemical predictors of adult growth hormone deficiency (#165)

Nayomi Perera 1 , John M Wentworth 1 , Christopher J Yates 1 2 , Angeline JJ Shen 1 , Cherie Chiang 1
  1. Diabetes and Endocrinology, Royal Melbourne Hospital, Parkville, VIC, Australia
  2. Department of General Medicine , Melbourne University , Parkville, Victoria, Australia

To access growth hormone (GH) replacement on the Australian Pharmaceutical Benefits Scheme, adults with acquired pituitary insufficiency must undergo stimulation testing to confirm GH deficiency (GHD), however US Endocrine Society Guidelines recommend that the presence of deficiencies in three or more pituitary axes strongly suggests the presence of GHD, and in this context provocative testing is optional [1,2]. The commonly performed glucagon stimulation test (GST) takes up to five hours and requires admission to an ambulatory treatment ward. We aimed to identify clinical and biochemical predictors of GHD that could be used to simplify or shorten GST duration [3].

 A prospective observational study of  GST performed at Royal Melbourne Hospital from January 2019 to June 2023 was conducted and approved by local ethics committee.

Of the 94 GST performed, , n=74 (79%) were consistent with  GHD . Abnormal GST  was  associated with deficiency of ≥2  pituitary hormone axes (p<0.0005), resected macroadenomas >1cm (p<0.05) and prior cranial irradiation (p =0.02). All 27 individuals with undetectable baseline fasting GH had GHD, while six patients had fasting GH above the stimulation threshold of 3.0mg/l, thereby had normal GH axis and did not require GST. Receiver operator curve analysis of the ability of fasting GH to predict GHD returned an area under the curve of 0.902 (95% CI 0.8230 – 0.9818, p<0.0001). The combination of ≥ 2 pituitary hormone deficiencies and fasting GH <0.5ug/L had a positive predictive value of 98% for diagnosis of GHD.

Measurement of fasting GH has potential to identify individuals with GH sufficiency, thereby obviating the need for prolonged, costly stimulation testing. The combination of fasting GH  and pituitary hormone deficiencies predicted GHD in our centre, work has commenced to verify this finding in an independent cohort as well as in the paediatric setting

 

  1. Mark E. Molitch, David R. Clemmons, Saul Malozowski, George R. Merriam, Mary Lee Vance (2011). Evaluation and treatment of adult growth hormone deficiency: An Endocrine Society Clinical Practice Guideline. JCEM. https://www.endocrine.org/clinical-practice-guidelines/adult-growth-hormone-deficiency
  2. Hartman ML, Crowe BJ, Biller BM, Ho KK, Clemmons DR, Chip- man JJ 2002 Which patients do not require a GH stimulation test for the diagnosis of adult GH deficiency? J Clin Endocrinol Metab 87:477– 485
  3. Chiang C, Inder W, Grossmann M, Clifton-Bligh R, Coates P, Lim EM, Ward P, Stanford P, Florkowski C, Doery J. (2021) Glucagon stimulation test. Harmonisation of Endocrine Dynamic Testing - Adult (HEDTA). The Endocrine Society of Australia and The Australasian Association of Clinical Biochemists, Australia. http://www.endocrinesociety.org.au/guidelines.asp