Heat stress is already a significant economic and welfare concern in Australia’s animal industries. However, there is limited evidence of the male’s contribution to the economic impacts, despite being well established that heat stress reduces male reproductive function. The influences of heat stress on males have been attributed to the impact that hot conditions play on sperm production. Increased testicular temperature has profound impacts on sperm quality, including decreased sperm concentration and motility, increased morphological abnormalities, altered plasma membrane composition and reduced DNA integrity. The effects of increased testicular temperature on male reproduction are not immediately apparent, where abnormal sperm first appear in the ejaculate 1 to 2 weeks post-heat insult. Previous studies have demonstrated that heat stress can negatively impact male reproductive function. These studies have predominantly utilised a scrotal insulation technique, which directly impedes the thermoregulatory capacity of the scrotum. This technique also precludes the capacity of the behavioural and physiological responses that occur in an animals attempt to mitigate the impact of heat stress on the scrotum, resulting in an isolated heat challenge. This is an important concept as it removes the capability of the whole body to effectively evoke the conserved responses for coping with heat load conditions. As such, there are limited studies investigating the impact of heat stress on male reproductive function when the whole-body has been exposed to heat load. With this in mind, we developed a protocol for assessing scrotal thermoregulation in bulls during whole-body exposure to heat load conditions. We identified that the thermoregulatory capabilities of the scrotum are challenged during whole body exposure to heat load conditions. In the context of climate change, it is probable that there will be more persistent challenges placed on the thermoregulatory capabilities of the scrotum, increasing the deleterious effects of heat stress on males.